Tehran University of Medical Sciences Nursing Practice Today 2383-1154 12 3 2025 04 23 285 294 Omar Abdalhafith Adult Health Nursing Department, Faculty of Nursing, Jordan University of Science and Technology, Irbid, Jordan Mohammad Rababa Adult Health Nursing Department, Faculty of Nursing, Jordan University of Science and Technology, Irbid, Jordan AND College of Nursing, Sulaiman AlRajhi University, Al Bukayriah, Saudi Arabia Audai Hayajneh Adult Health Nursing Department, Faculty of Nursing, Jordan University of Science and Technology, Irbid, Jordan 2024 11 23 2025 03 10 Background & Aim: Sepsis is a major global health issue, often leading to delayed recognition due to its varied symptoms. ICU nurses play a crucial role in early detection, but knowledge gaps in sepsis assessment and management persist. This study aimed to examine critical care nurses’ knowledge and experience of caring for patients with sepsis. Methods & Materials: A cross-sectional correlational study was conducted on a convenience sample of 320 ICU nurses from various hospitals in Jordan. A Validated tool assessed nurses’ knowledge and experience of sepsis management. Results: Nurses demonstrated moderate knowledge, with an overall score of 9.76 ± 1.43. Hypotension (51.6%) was the most recognized symptom, and 94.7% understood sepsis involves an infection with an inflammatory response. However, knowledge gaps remained, with only 31.9% knowing antibiotics should be given within 2 hours and 73.4% recognizing they should be administered before blood cultures. Regarding SIRS criteria, only a small percentage correctly identified key signs, such as temperature <36°C (26.3%) and heart rate >80 bpm (23.8%). Sepsis risk factors like IV drug use (34.4%) and chronic steroid use (21.9%) were recognized, but fewer identified the elderly (10.6%) as high-risk. Hypotension (50.9%) was the most challenging aspect of care. Nurses emphasized the need for better education (50.6%) and improved treatment protocols (46.9%). Conclusion: This study highlights critical gaps in ICU nurses’ knowledge and experience in assessing and managing sepsis. The findings identified region-specific barriers and the urgent need for tailored training programs in resource-limited critical care settings. https://npt.tums.ac.ir/index.php/npt/article/view/3650 https://npt.tums.ac.ir/index.php/npt/article/download/3650/662